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Is it true that single-stranded DNA libraries are initially BOUND directly to the flow cell as suggested in Illumina's diagrams (see panel b in attached figure). I always assumed they simply hybridized to the primer lawns.
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Yeah, that seems a little misleading. I would say that panel b more accurately represents the state after the initial cycle of amplification (primer extension). The flow cell bound primer has been extended using the hybridized library molecule template, then the original template strand has been denatured and washed away.Originally posted by drdna View Post
It seems the description of the initial stages in the this thread aren't quite accurate. -
This Illumina figure is also incorrect. As per my understanding, it is not possible to generate double stranded molecules with free ends!
from the link:
Last edited by drdna; 07-20-2015, 07:09 AM.Comment
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No, that's the original image that is also inaccurate for a different reason - see original post. It seems that the Illumina folks did not have anyone who understands the technology check the diagrams coming out of their illustration department/provider. I wanted to use an original Illumina illustration in a talk that describes the principle of bridge amplification but the official diagrams will confuse the audience, so I think I'll create my own.Originally posted by GenoMax View PostComment
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Try the original paper: Nucl. Acids Res. (2000) 28 (20): e87. doi: 10.1093/nar/28.20.e87. Figure 1.Originally posted by drdna View PostComment
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
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