Hi all,
Im currently building small RNA libraries from the same animal tissue, but after different treatments. We're trying to see if small RNAs in this tissue are differentially regulated because of the treatment. The problem im having is a normalization parameter. I thought of spiking-in constant amounts of a non- related plant or bacteriophage small RNA, into the total RNA from each treatment sample, and then making the libraries..
Has any one done this before? Or are there better ways to normalize your samples so that one can directly compare expression levels of small RNAs?
Appreciate your help!
Im currently building small RNA libraries from the same animal tissue, but after different treatments. We're trying to see if small RNAs in this tissue are differentially regulated because of the treatment. The problem im having is a normalization parameter. I thought of spiking-in constant amounts of a non- related plant or bacteriophage small RNA, into the total RNA from each treatment sample, and then making the libraries..
Has any one done this before? Or are there better ways to normalize your samples so that one can directly compare expression levels of small RNAs?
Appreciate your help!
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