I'm getting a significant high MW peak on a nano RNA chip with total RNA. Any thoughts on what this might be caused by? It is present in all the total RNA samples I loaded on this chip.
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Possibly genomic DNA. Have you digested your DNA and if so with what?
Some good info here:
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Did it help? You don't see this peak anymore?
I also have problem with this peak (though it is a bit smaller in my case). I am always denaturing my RNA prior to the run but maybe 2 min is not enough?
I think it is not DNA as second DNase treatment did not help and qPCR (+RT vs -RT, housekeeping gene) shows that the RNA is free of DNA (no amplification after 40 cycles). I also tried spiking in a small amount of gDNA and it never resulted in such electropherogram.
Then again I have seen this peak in many publications and examples of "good RNA".
http://www.sigmaaldrich.com/technica...ssessment.html fig 7.9
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4535892/ fig 1.
What can that be?
I am very puzzled.Attached Files
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