Hello,
I'm new to immunoprecipitation and DNA shearing. I would really appreciate any comment on the shearing I obtained on two samples I'm preparing (please see attached pdf for gel picture and other details). The downstream application will be Chip-seq sequencing by Illumina sequencer.
Some questions I have:
- Is the size of the smear acceptable for Chipseq?
- Having loaded up to 2 ug, the smear is quite weak. I think a lot of DNA has been shattered in small pieces that ran out of the gel. Is this worrying?
- On the Nanodrop, the two samples have nearly identical concentration (c.ca 360 ng/ul) and have been processed together. Still the smears look quite different on gel. Is this worrying?
Many thanks!
Dario
I'm new to immunoprecipitation and DNA shearing. I would really appreciate any comment on the shearing I obtained on two samples I'm preparing (please see attached pdf for gel picture and other details). The downstream application will be Chip-seq sequencing by Illumina sequencer.
Some questions I have:
- Is the size of the smear acceptable for Chipseq?
- Having loaded up to 2 ug, the smear is quite weak. I think a lot of DNA has been shattered in small pieces that ran out of the gel. Is this worrying?
- On the Nanodrop, the two samples have nearly identical concentration (c.ca 360 ng/ul) and have been processed together. Still the smears look quite different on gel. Is this worrying?
Many thanks!
Dario
Comment