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  • #1

    4C libraries

    Hi,
    We have a customer who asked if it is possible to sequence libraries made with the 4C protocol.
    The result of this protocol is a pcr product in the range from 2 kb to 100 bp. These pcr fragments are build up as follow:
    pcrprimer1-restrictionsite-knownsequence(7bp)-insert-restrictionsite-pcrprimer2
    These fragments have to stay intact, so no fragmentation possible.

    Did anybody ever tried this or has some good ideas?

    Thanks!
    Tags: None
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