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  • hewenjuan
    Junior Member
    • Sep 2017
    • 7
    #1

    Best magnet plate for beads separation

    Hello all,

    I am recently doing sequencing library prep in a 96-well plate. I found that especially with small volume, magnet beads separation can take very long. In addition, when I try to remove all the residual solution, I often get beads carryover. I wonder whether getting a better and stronger magnet plate will help. Any suggestions?
    The magnet plate I am using:
    Magnetic Stand-96
    https://www.thermofisher.com/order/catalog/product/AM10027
    For the paramagnetic bead precipitation from standard 96-well U-bottom microplates and 0.2 mL PCR plates with no additional accessories. The Ambion stand has a clear polyacryl base in a standard SBS 96-well microplate footprint. The


    Thanks!
    Tags: None
  • huguesparri
    Member
    • May 2008
    • 97
    #2
    Hello,
    Did you try the Agencourt SPRIPlate 96 (ref. A29164 in the beckman Coulter catalog)?
    We use it in our lab and we are rather satisfied with it.
    regards,

    Hugues

    Comment

    • thermophile
      Senior Member
      • Apr 2015
      • 243
      #3
      Magnum FLX® with Solid-Core™ Technology - Alpaqua
      https://www.alpaqua.com/Products/Magnet-Plates/Magnum-FLX
      Universal 96-well magnet plate containing proprietary Solid-Core ring magnets for rapid magnetic bead separation from sample volumes as large as 2 mL and elution in as little as 10 µl.  Compatible with all commonly used 96-well microplates and robotic platforms. Contains Spring Cushion Technology.


      by far the best plate mag I've tried. it's expensive but if you are doing lots of cleanups and want speed and small volumes it's probably worth it.
      Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.

      Comment

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