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  • Laiumiunix
    Junior Member
    • Apr 2018
    • 4

    #1

    ATAC-seq

    HI,

    I'm a little confused how to orders the primers too:
    Just to be sure if I understand:

    For each sample:

    I will need:

    Sample1
    Ad1_noMX:
    Ad2.1_index

    Sample2
    Ad1_noMX:
    Ad2.2_index:

    etc
    In terms to order the primers if I follow the list from (Jason D Buenrostro paper):

    I should be order:

    Ad1_noMX: AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG


    Basically the second primer I have to put the index sequence to order:
    index+sequence?
    Ad2.1_TAAGGCGA: TAAGGCGA-CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTCTCGTGGGCTCGGAGATGT


    Thanks

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