HI,
I'm a little confused how to orders the primers too:
Just to be sure if I understand:
For each sample:
I will need:
Sample1
Ad1_noMX:
Ad2.1_index
Sample2
Ad1_noMX:
Ad2.2_index:
etc
In terms to order the primers if I follow the list from (Jason D Buenrostro paper):
I should be order:
Ad1_noMX: AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG
Basically the second primer I have to put the index sequence to order:
index+sequence?
Ad2.1_TAAGGCGA: TAAGGCGA-CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTCTCGTGGGCTCGGAGATGT
Thanks
I'm a little confused how to orders the primers too:
Just to be sure if I understand:
For each sample:
I will need:
Sample1
Ad1_noMX:
Ad2.1_index
Sample2
Ad1_noMX:
Ad2.2_index:
etc
In terms to order the primers if I follow the list from (Jason D Buenrostro paper):
I should be order:
Ad1_noMX: AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG
Basically the second primer I have to put the index sequence to order:
index+sequence?
Ad2.1_TAAGGCGA: TAAGGCGA-CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTCTCGTGGGCTCGGAGATGT
Thanks