Hi,
I'm working with the AMPure beads to purify my library preparation products after ligation and PCR steps following the manufactors protocol. But its not possible to get rid of the adapter dimers.Now I hear that you have to calibrate the beads before using it. Is that rigth? And how should I do that?
May be its a naiv question, but I can't find any protocol or help!
Thank you.
I'm working with the AMPure beads to purify my library preparation products after ligation and PCR steps following the manufactors protocol. But its not possible to get rid of the adapter dimers.Now I hear that you have to calibrate the beads before using it. Is that rigth? And how should I do that?
May be its a naiv question, but I can't find any protocol or help!
Thank you.
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