Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • How to resuspend Ampure beads which fail to resuspend?

    I drammatically overdried my Ampure beads, now even pipetting over and over I cannot resuspend them: the lie at the bottom of the tube in large clumps. Any idea? I am afraid I cannot mess this experiment ;(

  • #2
    Give it more time and vortex once in a while. They will resuspend in the end.

    Comment


    • #3
      I'm late to the party, but alternatively, wash with ethanol again and vortex well. The ethanol should remove most of the air trapped in the precipitate, and at that point you could dry again and resuspend.

      If you tried luc's suggestion, I'd be happy to know if it worked out too!

      Comment


      • #4
        I agree on just incubating longer - I put my samples on the thermomixer with intermittent mixing until they dissolve. It works fine

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Best Practices for Single-Cell Sequencing Analysis
          by seqadmin



          While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
          Yesterday, 07:15 AM
        • seqadmin
          Latest Developments in Precision Medicine
          by seqadmin



          Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

          Somatic Genomics
          “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
          05-24-2024, 01:16 PM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Today, 06:58 AM
        0 responses
        13 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, Yesterday, 08:18 AM
        0 responses
        19 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, Yesterday, 08:04 AM
        0 responses
        18 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 06-03-2024, 06:55 AM
        0 responses
        13 views
        0 likes
        Last Post seqadmin  
        Working...
        X