Correct Ratio for two-sided PCR-cleanup with Ampure XP beads

[bheida]

Hey guys,
I want to use Ampure XP beads for size selection after PCR. I tried to find the right ratio of beads : pcr-product but got mixed results so far.

I'm curious, what ratios should work approximately for a two-sided clean-up if i want to select pcr-products around 170-230bp.

I would like to have a very clean product. The PCR is run with genomic DNA which has been sheared to a length of around 10000bp and the Primers used are around 55bp. Both should be cleaned out as well as possible.

Thanks for the input.

[nucacidhunter]

Double SPRI size selection window will be much larger than 50 bp. For your target you need to use Sage Pippin or gel extraction.

[JakobHedegaard]

Take a look at https://ls.beckmancoulter.co.jp/file...SPRIselect.pdf
But I agree with nucacidhunter that 50 bp is a too narrow window for a double-SPRI