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Hi!
Does anyone here have experience with single-stranded library preparation of genomic DNA?
We have recently whole genome sequenced FFPE-derived DNA with "standard" double-stranded preparation methods, and the data coverage has been quite poor (due to low yield and lack of complexity issues). I have understood that ss-methods have been widely used for sequencing of ancient DNA, and also that they might outperform ds-methods when sequencing FFPE-derived DNA. E.g. https://academic.oup.com/nar/article/45/10/e79/2948436. Can anyone confirm that this is the case? Are there some specific issues with this kind of prep?
Thanks in advance.
Does anyone here have experience with single-stranded library preparation of genomic DNA?
We have recently whole genome sequenced FFPE-derived DNA with "standard" double-stranded preparation methods, and the data coverage has been quite poor (due to low yield and lack of complexity issues). I have understood that ss-methods have been widely used for sequencing of ancient DNA, and also that they might outperform ds-methods when sequencing FFPE-derived DNA. E.g. https://academic.oup.com/nar/article/45/10/e79/2948436. Can anyone confirm that this is the case? Are there some specific issues with this kind of prep?
Thanks in advance.
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