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  • #1

    please help me to figure out what's wrong in my Ribomus library preparation

    Hi all,

    I used TruSeq stranded total RNA sample preparation guide to remove ribosomal RNA. However, my final library had a high peak in bio-analyzer data (as attached file).

    My condition list below:
    RIN of RNA: 10
    input RNA: 1 ug
    digest time: 8 mins as protocol
    another condition was by the protocol.

    Could anyone help me to figure out which step I should modify?

    Many thanks.

    Aero Ma
    Attached Files
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