I recently obtained strange results from a ChIP-seq experiment. Rather than having relatively thorough coverage of the genome, my reads were separated by large gaps and many were identical to each other and stacked right on top of each other. (I should note that I did also see enrichment at predicted areas.) We have not observed this pattern before. Then only thing that we see abnormal in the Illumina sequencer output info is that there was a large bias for G as the first nucleotide in the sequences. Could be related to the large stacks of identical reads? Any thoughts on why this may be occuring? Do you think it could be an inefficient ligation or amplification issue? TIA!
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM