I recently obtained strange results from a ChIP-seq experiment. Rather than having relatively thorough coverage of the genome, my reads were separated by large gaps and many were identical to each other and stacked right on top of each other. (I should note that I did also see enrichment at predicted areas.) We have not observed this pattern before. Then only thing that we see abnormal in the Illumina sequencer output info is that there was a large bias for G as the first nucleotide in the sequences. Could be related to the large stacks of identical reads? Any thoughts on why this may be occuring? Do you think it could be an inefficient ligation or amplification issue? TIA!
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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07-08-2024, 03:19 PM -
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