Originally posted by Simone78
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I don't know if you noticed that the newly published TGIRT paper in the journal RNA. They use the TGIRT to do the RT of human plasma RNA. And I realized that there is a fragmentation step prior to RT when dealing with whole cell total RNA as they suggested. Thus, on my perspective, defeat its better processivity compared to retroviral RT. You mentioned that the average size of cDNA generated by TGIRT is 4-5k. I just wonder if you have an in-house protocol to do so. It would be much appreciated if you let me know.
BTW, I noticed that the TGIRT can template switch not only RNA but also DNA, which means it might not be compatible with single-cell RNA-seq, unless we extract the total RNA from a single cell and do rRNA-depletion before RT, am I right?
Best!
Gary
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