Hello Everyone,
I am new at RNA-seq, switching from the good old Genechip to this technology... We have very limited RNA input amounts to play with and since we've been happy with Nugen amplification system in the past, so we're planning on using the 3'DGE amplification kit.
My questions are in regard of Nugen's Encore NGS library prep kit. We're going to do single-end sequencing for differential gene expression analysis. We have a ref genome so we don't really care about gene coverage and such. In addition, I received a bit of conflicting advice about cDNA fragmentation, or the need to do it. Any thoughts on the matter?
thanks!
I am new at RNA-seq, switching from the good old Genechip to this technology... We have very limited RNA input amounts to play with and since we've been happy with Nugen amplification system in the past, so we're planning on using the 3'DGE amplification kit.
My questions are in regard of Nugen's Encore NGS library prep kit. We're going to do single-end sequencing for differential gene expression analysis. We have a ref genome so we don't really care about gene coverage and such. In addition, I received a bit of conflicting advice about cDNA fragmentation, or the need to do it. Any thoughts on the matter?
thanks!
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