Wonder if we can learn something about reference mapping here. We have scRNA-seq data form 10x platform. We want to map barcodes to mouse immune cells. When we will use two references, we got very different results regarding fractions of immune cells. Using one reference, we see more macrophages than dendritic cells; using another reference, we see more dendritic cells than dendritic cells. I am sure someone here may have similar experience? What are your opinions or solutions? Thanks.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM