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  • kasthuri
    Member
    • Jun 2011
    • 36

    #1

    Mapping SOLiD Reads using BFast.

    I am new to using BFast. I was able to successfully install it. I now
    have question regarding mapping the reads.

    I have two SOLiD csfasta paired end files,

    reads1.csfasta - 50 bp
    reads2.csfasta - 35 bp

    What is best way to align them using BFast for the human genome? If I
    am using two separate commands how to merge them to obtain a single
    bam/sam file?

    I would want to use GATK pipeline for making SNP calls and so how do I
    make sure that the aligned reads has good default base quality?

    I would greatly appreciate the reply.

    Thanks
    -Kasthuri

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