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  • akolman
    Junior Member
    • Jun 2011
    • 7

    GEOquery error message

    Hello,

    I am using Windows 7 and R 2.12.2

    When I try to run these two lines I get the following error from getGEO

    library(GEOquery)
    GSE7562 <- getGEO("GSE7562")

    Found 1 file(s) GSE7562_series_matrix.txt.gz Error in file(con, "r") : cannot open the connection In addition: Warning messages: 1: In download.file(sprintf("ftp://ftp.ncbi.nih.gov/pub/geo/DATA/SeriesMatrix/%s/%s", : download had nonzero exit status 2: In file(con, "r") : cannot open file 'C:\Users\APD\AppData\Local\Temp\RtmptmeDAb/GSE7562_series_matrix.txt.gz': No such file or directory

    Am I missing something to get GEOquery working?

    Thanks in advance,
  • maubp
    Peter (Biopython etc)
    • Jul 2009
    • 1544

    #2
    Can you get to this file manually, e.g. in your browser:
    ftp://ftp.ncbi.nih.gov/pub/geo/DATA/..._matrix.txt.gz

    That is what I believe geoquery is trying to download. Its about 2.5MB so quite small. I'm finding the FTP site works but is a bit slow....

    Comment

    • akolman
      Junior Member
      • Jun 2011
      • 7

      #3
      Thanks. I downloaded the file but I still have the same error. Then, I went to the GEO Accession viewer and downloaded the file in .soft format. That seems to work. I would be a little disappointed if I have to these all the time.

      Comment

      • maubp
        Peter (Biopython etc)
        • Jul 2009
        • 1544

        #4
        What version of geoquery do you have? I notice your R is a little out of date.

        Comment

        • akolman
          Junior Member
          • Jun 2011
          • 7

          #5
          I have version 2.17.5

          Comment

          • maubp
            Peter (Biopython etc)
            • Jul 2009
            • 1544

            #6
            The current version is geoquery 2.18.0, that might fix this...
            The NCBI Gene Expression Omnibus (GEO) is a public repository of microarray data. Given the rich and varied nature of this resource, it is only natural to want to apply BioConductor tools to these data. GEOquery is the bridge between GEO and BioConductor.

            Comment

            • akolman
              Junior Member
              • Jun 2011
              • 7

              #7
              This is somewhat unrelated. How can I update R and without losing all the packages I have installed?

              I tried installing the newer version of GEOquery but it does work with my current R version.

              Comment

              • maubp
                Peter (Biopython etc)
                • Jul 2009
                • 1544

                #8
                Originally posted by akolman View Post
                This is somewhat unrelated. How can I update R and without losing all the packages I have installed?
                I don't think you can - the R API is likely to change between major releases. There might be a neat way to get a list of all the installed libraries, to make re-installing the same set easier - they can be installed via the R command line as well as via the R GUI.
                Originally posted by akolman View Post
                I tried installing the newer version of GEOquery but it does work with my current R version.
                Try asking Sean Davis directly about this error. Also, if it was a network problem, it might go away on its own - have you re-tried at a different time of day?

                Comment

                • akolman
                  Junior Member
                  • Jun 2011
                  • 7

                  #9
                  I updated R and installed the newest version of GEOquery, and I still get the same error message. Anyway, I think I can get away not using the getGEO function to download the files.

                  Comment

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