Has anybody gotten this to work using windows? If not, are there any ways to convert sra to fastq for someone on a windows OS? It seems that Galaxy has nothing regarding sra file formats.
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I think I got it
I went back to NCBI website and found a ncbi sra toolkit designed for 32 bit windows os. It seems to be working now. -
Use SRA to do conversion?
A few months ago Stephen Taylor suggested using the SRA's conversion itself:
The way I got round this was let the SRA website do the conversion. So just paste in the URL in the upload screen:
Where SRRXXXXXX is the archive of interest, and it downloads Sanger FASTQ sequences to Galaxy.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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