I've been trying to convert my .csfasta and .qual files into a .fastq file for the last two days. I've found two different solid2fastq scripts (one from bfast, I don't remember the other one - might be a link from seqanswers). When I look at my .csfasta file, I see the I have 51 nucleotides for each read (they all start with T for the adapter) and there are 50 characters for each read in my .qual file. When I try to trim one base from the .csfasta files, both scripts trim one character from the quality scores too so my .fastq file has one missing quality score and downstream programs don't like this. What should I use for my conversion that would trim one base from .csfasta but not from .qual?
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ahmetz,did you successfully applied FusionMap on your Solid data? I want to find fusion genes with Solid RNA-seq data, too. And How does FusionMap aligner work, can it built colorspace index? Can you give me email address for more communication? My Email is [email protected],Thank you!
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