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  • Phagealign for Paired-End analysis

    Hi everybody,
    is it possible to use Phagealign for Paired-End analysis? I know it is much slower than Eland and so on...But we would like to compare the results for a specific application which deals with a very small genome. If it is possible, what would be the right GERALD parameter (For Eland it is "ANALYSIS eland_pair")?
    Thanks and best,
    Chris

  • #2
    Did u try using
    Code:
    ANALYSIS default
    ? I haven't used it but looks like the same for Single as well as pair end.

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    • #3
      No, you cannot use phageAlign for paired ends -- the pairing scripts run off the eland_multi output and will not accept the phageAlign output. You can however analyze each end separately -- do an "ANALYSIS default" run with "USE_BASES as Y36N36 and then a second run with N36Y36.

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      • #4
        Thanks a lot for your replies.
        Chris

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