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  • cbasel
    Junior Member
    • Mar 2009
    • 2

    #1

    Phagealign for Paired-End analysis

    Hi everybody,
    is it possible to use Phagealign for Paired-End analysis? I know it is much slower than Eland and so on...But we would like to compare the results for a specific application which deals with a very small genome. If it is possible, what would be the right GERALD parameter (For Eland it is "ANALYSIS eland_pair")?
    Thanks and best,
    Chris
  • seq_GA
    Senior Member
    • Feb 2009
    • 124

    #2
    Did u try using
    Code:
    ANALYSIS default
    ? I haven't used it but looks like the same for Single as well as pair end.

    Comment

    • dcjamison
      Member
      • Oct 2008
      • 15

      #3
      No, you cannot use phageAlign for paired ends -- the pairing scripts run off the eland_multi output and will not accept the phageAlign output. You can however analyze each end separately -- do an "ANALYSIS default" run with "USE_BASES as Y36N36 and then a second run with N36Y36.

      Comment

      • cbasel
        Junior Member
        • Mar 2009
        • 2

        #4
        Thanks a lot for your replies.
        Chris

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