Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • cbasel
    Junior Member
    • Mar 2009
    • 2

    #1

    Phagealign for Paired-End analysis

    Hi everybody,
    is it possible to use Phagealign for Paired-End analysis? I know it is much slower than Eland and so on...But we would like to compare the results for a specific application which deals with a very small genome. If it is possible, what would be the right GERALD parameter (For Eland it is "ANALYSIS eland_pair")?
    Thanks and best,
    Chris
  • seq_GA
    Senior Member
    • Feb 2009
    • 124

    #2
    Did u try using
    Code:
    ANALYSIS default
    ? I haven't used it but looks like the same for Single as well as pair end.

    Comment

    • dcjamison
      Member
      • Oct 2008
      • 15

      #3
      No, you cannot use phageAlign for paired ends -- the pairing scripts run off the eland_multi output and will not accept the phageAlign output. You can however analyze each end separately -- do an "ANALYSIS default" run with "USE_BASES as Y36N36 and then a second run with N36Y36.

      Comment

      • cbasel
        Junior Member
        • Mar 2009
        • 2

        #4
        Thanks a lot for your replies.
        Chris

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by SEQadmin2





          NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
          10-30-2026, 06:46 AM
        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
          ...
          10-14-2026, 09:55 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, Today, 07:59 AM
        0 responses
        13 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-05-2026, 09:25 AM
        0 responses
        32 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 11-03-2026, 11:54 AM
        0 responses
        45 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-28-2026, 09:57 AM
        0 responses
        169 views
        0 reactions
        Last Post SEQadmin2  
        Working...