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  • Error Message for read length

    Getting this error:
    Parameter read.length=35 does not agree with read file sequence length=50

    I changed the read.length back to 50, but then it says the opposite:
    "does not agree with read file sequence length=35"

    Here is my ini file:

    import global/global.ini
    run_name = arupaRun1
    sample_name = arupaSample1

    reference = ${reference.dir}/ch11_12_validated.fasta
    read.length = 35

    mapping.run = 1
    mapping.tagfiles.dir = ${reads.result.dir.1}
    mapping.output.dir = ${output.dir}/chr
    mismatch.level = 2

    Any suggestions?

  • #2
    What software? What was your command? Do you have a test case?

    Comment


    • #3
      I am running bioscope. I used 2 different ini files for two csfasta/qual reads. I keep getting this error. I have no test case. First time running bioscope.

      Comment


      • #4
        its a 50bp x 35bp paired-end if that helps

        Comment

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