Originally posted by vebaev
View Post
-
If you have 0 counts in one condition and hundreds of counts in other, this is most likely a valid signal, and DESeq should indicate this with a small p value. Of course, the fold change estimate is not to useful but that is a general problem if one of conditions has very low counts -
Yes Simon,
the =Inf are coming from rows where one of the groups are 0 and other is some reads (sometimes 5 simetimes 500)
I also found your post in other topic about these =Inf that if the last 2 columns the values are too big or close to zero I should discard these rows from further analysis?Last edited by vebaev; 08-19-2011, 12:43 AM.------------
SMART - bioinfo.uni-plovdiv.bgComment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment