How I find not assembly read in a reference assembly???

[matiasfreired]

Hello everyone!

I made a sequence of 454 junior GS, I want to make comparisons with the reference genome. I am interested in any delesion or insertion into the genome sequenced, did the assembly with the reference genome with the DNAstar program, but do not know how I can find the readings that were not assembled. Precisely, these are sequences that interest me, somebody help me??

thanks in advance!!

[seb567]

Hello everyone!

I made a sequence of 454 junior GS, I want to make comparisons with the reference genome. I am interested in any delesion or insertion into the genome sequenced, did the assembly with the reference genome with the DNAstar program, but do not know how I can find the readings that were not assembled. Precisely, these are sequences that interest me, somebody help me??

thanks in advance!!

Hello,

Perhaps you can contact DNAstar, I am sure they will help.

There is something, however, that I don't quite grasp in your query. How come you want to fetch unaligned sequences while your primary goal, as you say, is any "deletion or insertion into the genome sequenced."


Sébastien Boisvert