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  • sdvie
    Member
    • Jul 2010
    • 68

    #1

    duplicate entries in pileup file?

    When greping for positions of interest, I noticed that my pileup file seems to contain a number of lines that repeat chromosome number and position, like this:

    Code:
    chr11	67048294	T	T	93	0	60	25	,$,$..,,,,,,,,,,,,,,,,,,,,,-2gc	EEOPPPMTT_]<ETNT3,=>%.7/!	~~~~~~~~~~~~~~~~~~~~~~~~~
    chr11	67048294	*	*/*	37	0	60	25	*	-GC	24	1	0	0	0
    From a pileup of a total of 15980465 lines, I get 10346 not-unique positions (chr + position).
    Is this normal? If yes, why does this happen? And if not, what might be the reason?

    Many thanks in advance for your comments.
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    That's absolutely normal behavior for pileup when there's an indel in one of the reads.

    Pileup has been deprecated for a long time now. Use mpileup instead.

    Comment

    • sdvie
      Member
      • Jul 2010
      • 68

      #3
      Thanks, that is good to know.

      I am aware that pileup has been replaced by mpileup, however, I was reproducing data generated with the old pileup, when I came about this issue.

      Cheers!

      Comment

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