Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • sdvie
    Member
    • Jul 2010
    • 68

    #1

    duplicate entries in pileup file?

    When greping for positions of interest, I noticed that my pileup file seems to contain a number of lines that repeat chromosome number and position, like this:

    Code:
    chr11	67048294	T	T	93	0	60	25	,$,$..,,,,,,,,,,,,,,,,,,,,,-2gc	EEOPPPMTT_]<ETNT3,=>%.7/!	~~~~~~~~~~~~~~~~~~~~~~~~~
    chr11	67048294	*	*/*	37	0	60	25	*	-GC	24	1	0	0	0
    From a pileup of a total of 15980465 lines, I get 10346 not-unique positions (chr + position).
    Is this normal? If yes, why does this happen? And if not, what might be the reason?

    Many thanks in advance for your comments.
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    That's absolutely normal behavior for pileup when there's an indel in one of the reads.

    Pileup has been deprecated for a long time now. Use mpileup instead.

    Comment

    • sdvie
      Member
      • Jul 2010
      • 68

      #3
      Thanks, that is good to know.

      I am aware that pileup has been replaced by mpileup, however, I was reproducing data generated with the old pileup, when I came about this issue.

      Cheers!

      Comment

      Latest Articles

      Collapse

      • SEQadmin2
        Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
        by SEQadmin2



        Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

        Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
        ...
        10-14-2026, 09:55 AM
      • SEQadmin2
        New Genomics Technologies Take Aim at Long-Standing Limits
        by SEQadmin2


        Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

        We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
        ...
        09-28-2026, 10:25 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, Yesterday, 11:13 AM
      0 responses
      14 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, Yesterday, 10:48 AM
      0 responses
      13 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-15-2026, 10:52 AM
      0 responses
      35 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 10-14-2026, 05:39 AM
      0 responses
      42 views
      0 reactions
      Last Post SEQadmin2  
      Working...