I am a biologist and a poor programmer. I have assembled RNA-seq data from which I'd like to extract all potential long ncRNA candidates. I assume the easiest way would be to compare my data to Refseq or Rfam but I don't know how to do this. Furthermore, this strategy would only pick up already annotated lncRNA. I would be glad of any help.
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Last edited by stephenhart; 09-28-2011, 11:45 PM. -
You can use Dario web server which is a free web tool for ncRNA analysis using RNAseq data.
I have not used it before. so can not comment on the results. Please let me know your experience wit it. -
You could do it with Annocript. It annotates the transcriptome and gives putative lnc RNAs sequences...Comment
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
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