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Originally posted by paulorapazote View Post
I got 100 samples to analyse so it would be useful and efficient if I can run it on server. I can process using the command line but the output of astalavista was in binary which I can't open it. Would you have any idea?Comment
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Sorry, I never worked with the command line. I briefly checked the 2.2 version and command is
./astalavista.sh -c asta -i <input GTF> -o <output file>Comment
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Originally posted by paulorapazote View Post
The problem was the output file is a binary file. There was no command to specify the output. I tried the webserver as you suggest but end up no result page after they finished processing my GTF file. I contacted the admin and webmaster but no answer yet from them.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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