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  • lewewoo
    Member
    • Apr 2011
    • 60

    #1

    Help: how to handle .bam file

    to do some analysis of reads, I have first converted .bam to .sam then write a perl script; however, I would like to directly operate .bam because of its smaller size. Anybody have experiences on writing codes to handle .bam? Would it be possible to share the experience? Thanks!
  • Bukowski
    Senior Member
    • Jan 2010
    • 388

    #2
    Originally posted by lewewoo View Post
    to do some analysis of reads, I have first converted .bam to .sam then write a perl script; however, I would like to directly operate .bam because of its smaller size. Anybody have experiences on writing codes to handle .bam? Would it be possible to share the experience? Thanks!

    Comment

    • simonandrews
      Simon Andrews
      • May 2009
      • 870

      #3
      ..or simply replace:

      open (IN,'myfile.sam')...

      with:

      open (IN,'samtools view myfile.bam |')...

      ..and keep using the script you already have.

      Comment

      • lewewoo
        Member
        • Apr 2011
        • 60

        #4
        Thanks a lot!

        Comment

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