to do some analysis of reads, I have first converted .bam to .sam then write a perl script; however, I would like to directly operate .bam because of its smaller size. Anybody have experiences on writing codes to handle .bam? Would it be possible to share the experience? Thanks!
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Originally posted by lewewoo View Postto do some analysis of reads, I have first converted .bam to .sam then write a perl script; however, I would like to directly operate .bam because of its smaller size. Anybody have experiences on writing codes to handle .bam? Would it be possible to share the experience? Thanks!
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..or simply replace:
open (IN,'myfile.sam')...
with:
open (IN,'samtools view myfile.bam |')...
..and keep using the script you already have.
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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