We have sequenced a transcriptome of a species who does not have a sequenced genome, using 454 and our initial goal is to find a set of ESTs that represent genes. The 454 reads were assembled using Newbler 2.5 and the initial assembly gave ~26000 isotigs and 18,000. Contigs. After talking to the several people, I used CD-Hits program to combine the isotigs, contigs and singltons that were not assembled. After combining these sequences, I got ~4000 isotigs, ~17,000 contigs and ~30, 000 Singlton that were not assembled. Is this the correct way to do this? I couldn’t find any publication that has mentioned this method.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...Yesterday, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM -
Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...07-09-2026, 11:10 AM