You can use "NOISeq-sim: no replicates"
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Not much chance of statistical analysis with only two samples.Originally posted by feng View Post
I think DESeq http://www-huber.embl.de/users/anders/DESeq/ has instructions for unreplicated conditions though. If you search the forums there are plenty of discussions on SeqAnswers about this topic.Leave a comment:
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statistical analysis
How to do statistical analysis on differentially expressed genes from RNA-seq?
Results as
genes Sample1 Sample2
xxxx 12 23
xxxx 155 30
Numbers stand for times of gene sequence.
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