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  • feng
    Member
    • Oct 2010
    • 50
    #1

    statistical analysis

    How to do statistical analysis on differentially expressed genes from RNA-seq?

    Results as


    genes Sample1 Sample2
    xxxx 12 23
    xxxx 155 30

    Numbers stand for times of gene sequence.
    Tags: None
  • Bukowski
    Senior Member
    • Jan 2010
    • 388
    #2
    Originally posted by feng View Post
    How to do statistical analysis on differentially expressed genes from RNA-seq?

    Results as


    genes Sample1 Sample2
    xxxx 12 23
    xxxx 155 30

    Numbers stand for times of gene sequence.
    Not much chance of statistical analysis with only two samples.

    I think DESeq http://www-huber.embl.de/users/anders/DESeq/ has instructions for unreplicated conditions though. If you search the forums there are plenty of discussions on SeqAnswers about this topic.

    Comment

    • ickou
      Junior Member
      • Aug 2010
      • 8
      #3
      You can use "NOISeq-sim: no replicates"
      tutorial [NOISeq]
      http://bioinfo.cipf.es/noiseq/doku.php?id=tutorial
      tutorial

      Comment

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