I have moved to trimmomatic - it does SE and PE as well and in my hands much much faster.
Unconfigured Ad
Collapse
X
-
FAR does SE/PE as well (starting from version 2 AFAIK).Originally posted by kga1978 View PostI have moved to trimmomatic - it does SE and PE as well and in my hands much much faster.
Speed is not really a matter for me. If it takes five minutes per lane, fine, if it takes one hour, fine too (considering instrument run time and time for downstream analysis) :-)
Have you compared the results? Similar?
Comment
-
I'm using fastq-join and fastq-mcf. The first for overlapping paired end reads, the second to de-multiplex NuGEN RNA-seq libraries.Originally posted by sklages View PostYes, parameters and/or working principles are poorly documented. I agree, it is really fast.
Are you using fastq-mcf in production?
I'm trying to get some more doc (the developer is very active...)
Comment
Latest Articles
Collapse
-
by SEQadmin2
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...-
Channel: Articles
10-14-2026, 09:55 AM -
ad_right_rmr
Collapse
News
Collapse
| Topics | Statistics | Last Post | ||
|---|---|---|---|---|
|
Genome Sequencing Reveals Large, Repeat-Rich DNA in Trapdoor Spider Named for Stephen Colbert
by SEQadmin2
Started by SEQadmin2, Yesterday, 09:57 AM
|
0 responses
16 views
0 reactions
|
Last Post
by SEQadmin2
Yesterday, 09:57 AM
|
||
|
Started by SEQadmin2, Yesterday, 09:51 AM
|
0 responses
11 views
0 reactions
|
Last Post
by SEQadmin2
Yesterday, 09:51 AM
|
||
|
Started by SEQadmin2, 10-22-2026, 11:13 AM
|
0 responses
65 views
0 reactions
|
Last Post
by SEQadmin2
10-22-2026, 11:13 AM
|
||
|
Started by SEQadmin2, 10-22-2026, 10:48 AM
|
0 responses
47 views
0 reactions
|
Last Post
by SEQadmin2
10-22-2026, 10:48 AM
|
Comment