I have sff files from 454 sequencing. How do I convert sff to fasta or fastq format?
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I've converted sff to fastq using sffinfo to generate the fasta and qual files. You can then combine them using the perl script on this page
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
to combine them into Sanger fastq. Your fasta and qual files should have the same basename for it to work.
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I am building a tool with graphical interface that will convert SFF to FASTQ and multifasta.Last edited by create.share; 06-03-2014, 01:11 AM.
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