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  • hohosharon
    Junior Member
    • Dec 2011
    • 4

    #1

    what is the mean of "deviation" in fastqc?

    Hi:
    I have a question from http://www.bioinformatics.bbsrc.ac.u...20Content.html
    I don't know what is the mean of "deviation" on GC distribution over all sequences.
    Can u give me definition of deviation ?
    Thanks.
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Wikipedia has a convenient definition of deviation. You can also read the article on standard deviation, for which there are translations in many languages.

    Comment

    • hohosharon
      Junior Member
      • Dec 2011
      • 4

      #3
      The sum of the "deviations" from the normal distribution represents more than 15% of the reads.

      I think here's "deviations" maybe is not the same mean of "standard deviations".
      I guess here's "deviations" is the difference of C count per read and Theoretical Distribution, so i want to check what is the mean of "deviation" in here.
      thanks

      Comment

      • fkrueger
        Senior Member
        • Sep 2009
        • 627

        #4
        This module tests how closely the observed GC distribution curve resembles a modeled normal distribution with the same overall GC content as found in the sample. If they deviate, i.e. are different, more than 15%, FastQC will throw a warning.

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