[Volklor]

same problem

I'm having the same problem with samse using bwa version 0.5.7:

[bns_restore_core] fail to open file 'FRA_genome/Fvesca.ann'. Abort!

I'm using Illumina reads, so the length should not be an issue. And the .ann file is actually present.

Any ideas?

[ppoudel]

Hi,

How does BWA handle the coordinated sorted fastq files (obtained from coordinated sorted bam file)? Do I need to shuffle the bam files before converting them to fastqs?