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So what is the update with this project https://github.com/nh13/samtools . Is it still faster than multi-threaded version of samtools ? Is this going to be a good substitute for Goby and Cram ?
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Has there been any success on mpileup speedup ? Is the parallelization by pthreads or MPI ?Comment
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Is the bcftools that come with samtools also multithreaded ?Comment
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There was no desire from the folks maintaining samtools to take over the development and add it. Sorry for the delayed response.Originally posted by narain View PostComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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