Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • nxtgenkid10
    Member
    • Feb 2011
    • 16

    #1

    bowtie paired end

    I had paired end illumina reads of a bacterial genome and i have the reference so i m trying to use bowtie to align the same . so first i made the index of reference and used the following commands

    bowtie2 /Reference/genome -1 /DATA/Read1.fastq -2 /DATA/Read2.fastq -S phix.sam

    But i couldnt give the -n and -l command
    Y is it so ? and how could i give them
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Originally posted by nxtgenkid10 View Post
    But i couldnt give the -n and -l command
    Y is it so ? and how could i give them
    Aren't they -N and -L in bowtie2?

    Comment

    • yumtaoist
      Member
      • Dec 2011
      • 10

      #3
      dpryan is right, the arguments are case sensitive

      Comment

      • nxtgenkid10
        Member
        • Feb 2011
        • 16

        #4
        thanks i will try

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
          ...
          10-14-2026, 09:55 AM
        • SEQadmin2
          New Genomics Technologies Take Aim at Long-Standing Limits
          by SEQadmin2


          Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

          We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
          ...
          09-28-2026, 10:25 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 10-22-2026, 11:13 AM
        0 responses
        42 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-22-2026, 10:48 AM
        0 responses
        33 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-15-2026, 10:52 AM
        0 responses
        44 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-14-2026, 05:39 AM
        0 responses
        45 views
        0 reactions
        Last Post SEQadmin2  
        Working...