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  • nxtgenkid10
    Member
    • Feb 2011
    • 16

    bowtie paired end

    I had paired end illumina reads of a bacterial genome and i have the reference so i m trying to use bowtie to align the same . so first i made the index of reference and used the following commands

    bowtie2 /Reference/genome -1 /DATA/Read1.fastq -2 /DATA/Read2.fastq -S phix.sam

    But i couldnt give the -n and -l command
    Y is it so ? and how could i give them
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Originally posted by nxtgenkid10 View Post
    But i couldnt give the -n and -l command
    Y is it so ? and how could i give them
    Aren't they -N and -L in bowtie2?

    Comment

    • yumtaoist
      Member
      • Dec 2011
      • 10

      #3
      dpryan is right, the arguments are case sensitive

      Comment

      • nxtgenkid10
        Member
        • Feb 2011
        • 16

        #4
        thanks i will try

        Comment

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