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  • bowtie paired end

    I had paired end illumina reads of a bacterial genome and i have the reference so i m trying to use bowtie to align the same . so first i made the index of reference and used the following commands

    bowtie2 /Reference/genome -1 /DATA/Read1.fastq -2 /DATA/Read2.fastq -S phix.sam

    But i couldnt give the -n and -l command
    Y is it so ? and how could i give them

  • #2
    Originally posted by nxtgenkid10 View Post
    But i couldnt give the -n and -l command
    Y is it so ? and how could i give them
    Aren't they -N and -L in bowtie2?

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    • #3
      dpryan is right, the arguments are case sensitive

      Comment


      • #4
        thanks i will try

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