Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • info_nowise
    Junior Member
    • Feb 2012
    • 4

    #1

    vcftools site filter using bedfile

    Hi all

    I am trying to filter my vcf file in vcftools using a bed file. I am consistently getting an output vcf with 0 sites retained. Following is the command line I used:

    vcftools --gzvcf myfile.vcf.gz --recode --bed bedfile.bed --out outfile

    The log file looks like this:
    VCFtools - v0.1.6
    (C) Adam Auton 2009
    Parameters as interpreted:
    --out PsvaMvd7a
    --recode
    --gzvcf PsvaMvd7.vcf.gz
    --bed CF2_probes_mod_sorted.bed

    Scanning PsvaMvd7.vcf.gz ...
    Currently scanning CHROM: MT
    Currently scanning CHROM: 38
    Currently scanning CHROM: 35
    Currently scanning CHROM: 36
    Currently scanning CHROM: 37
    Currently scanning CHROM: 33
    Currently scanning CHROM: 32
    Currently scanning CHROM: 26.......
    Keeping 1718454 entries (out of 1718454 read)
    Done
    Filtering sites by BED file
    Kept 14 out of 14 Individuals
    Kept 0 out of 1718454 Sites
    Error:No data left for analysis!

    My bed file looks like this:

    "track name=""tiled_region"" description=""NimbleGen Tiled Regions"""
    chr1 11398149 11398377
    chr1 11398197 11398414
    chr1 11398377 11398448
    chr1 11453954 11454357
    chr1 11453999 11454327
    chr1 11456016 11456508
    chr1 11456061 11456496
    chr1 11537417 11537695
    chr1 11537488 11537637

    Appreciate any help. Thanks.
    Last edited by info_nowise; 04-19-2012, 05:58 AM. Reason: added more info
  • swbarnes2
    Senior Member
    • May 2008
    • 910

    #2
    Are you 100% sure that's wrong?

    BEDTools can also get you the intersect between a .vcf and a .bed

    Comment

    • info_nowise
      Junior Member
      • Feb 2012
      • 4

      #3
      Yes, I am sure. However, I figured out the problem. I got rid of the letters 'chr' in the bed file, leaving only the numbers and vcftools worked!

      Comment

      • info_nowise
        Junior Member
        • Feb 2012
        • 4

        #4
        Thanks for the tip, though, swbarnes2!

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
          ...
          10-14-2026, 09:55 AM
        • SEQadmin2
          New Genomics Technologies Take Aim at Long-Standing Limits
          by SEQadmin2


          Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

          We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
          ...
          09-28-2026, 10:25 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 10-22-2026, 11:13 AM
        0 responses
        28 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-22-2026, 10:48 AM
        0 responses
        21 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-15-2026, 10:52 AM
        0 responses
        38 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-14-2026, 05:39 AM
        0 responses
        44 views
        0 reactions
        Last Post SEQadmin2  
        Working...