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  • Hena
    Member
    • Nov 2009
    • 19

    #1

    SeqPrep and discarding reads

    I've been testing SeqPrep (http://seqanswers.com/wiki/SeqPrep) for trimming adapters and merging reads. However I'm unsure why some reads get discarded. The command line is as follows:

    SeqPrep -f s_5_1_sequence.txt.gz -r s_5_2_sequence.txt.gz -1 s_5_1-trimmed.fastq.gz -2 s_5_2-trimmed.fastq.gz -A AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -B AGATCGGAAGAGCGTCGTGTAGGGAAAGAGT -s merged.fastq.gz -L 1 -6 -E alignment.txt.gz

    I'm assuming -L 1 would prevent removal of any reads (the help on command line differs abit from the wiki page). However reads still get removed. What causes this and what should I do to affect this?
  • jp.
    Senior Member
    • Jul 2013
    • 142

    #2
    any solution did you get ?

    Comment

    • Hena
      Member
      • Nov 2009
      • 19

      #3
      I found an answer to why it does discard reads even after minimum length.

      First off, awesome bit of software. I recently went looking for something that does exactly what your code does. I was very happy to find it. To the point-- It seems that SeqPrep will discard read ...


      Edit: If SeqPrep matches adapters but fails to do proper merge of reads, then it discards the reads. I suppose it would be possible to make a patch to prevent this functionality.
      Last edited by Hena; 08-18-2013, 11:29 PM.

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