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I am using fastx toolkit and i generated the stats file. However, my boxplot generated a .png file but ubuntu says: failed to load image. Is there anyway to solve this issue? I have image viewers like eog and gwenview but it still doesn't load.
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Just a thought... if you use the fastq_quality_boxplot_graph.sh with -p option, the file generated is not .png, but .ps, and can not be opened like a .png. -
So I need to convert the .ps which is assumed to be .png to jpg or some other format?Comment
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No, just remove the -p option, and the files created should be .png, which shouldn't give you any difficulties when opening.Originally posted by bioinfo_ View PostComment
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These may seem obvious but not sure if you have tried to check:
1. Is the .png file non-zero byte size?
2. If so have you tried to move it to some other machine to see if it opens there?Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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