I am new to tophat. so be gentle.
When I run tophat with a test yeast data set, I got an error message after "Checking reads". after 3 lines of reads info, it says:
Could someone help me to figure out what I did wrong?
When I run tophat with a test yeast data set, I got an error message after "Checking reads". after 3 lines of reads info, it says:
Code:
[FONT="Courier New"] ~/$ bin/tophat '/path/to/bowtie/indexes/yeast' '/path/to/sequence/seq.fastq' ... ... [Mon Aug 17 15:53:01 2009] Checking reads seed length: 33bp format: fastq quality scale: --phred33-quals [FAILED] Error: prep_reads not found on this system. Did you forget to include it in your PATH? [/FONT]
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