Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • liux
    Member
    • Mar 2009
    • 30

    #1

    TopHat question: prep_reads not found?

    I am new to tophat. so be gentle.

    When I run tophat with a test yeast data set, I got an error message after "Checking reads". after 3 lines of reads info, it says:


    Code:
    [FONT="Courier New"]
    ~/$ bin/tophat '/path/to/bowtie/indexes/yeast' '/path/to/sequence/seq.fastq' 
    ...
    ...
    [Mon Aug 17 15:53:01 2009] Checking reads
    	seed length:	 33bp
    	format:		 fastq
    	quality scale:	 --phred33-quals
    	[FAILED]
    Error: prep_reads not found on this system.  Did you forget to include it in your PATH?
    [/FONT]
    Could someone help me to figure out what I did wrong?
  • liux
    Member
    • Mar 2009
    • 30

    #2
    Whoops, prep_reads is another program in tophat that is needed. once I add '/home/user/bin' in the PATH, it worked.

    Comment

    • mortnyc
      Junior Member
      • Oct 2009
      • 1

      #3
      Hey there, I too have the same problem i'm ot sure to which path am I gonna have to add that '/home/user/bin'?

      Comment

      Latest Articles

      Collapse

      • kinpict.com
        Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by kinpict.com
        The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
        Today, 06:37 PM
      • SEQadmin2
        16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
        by SEQadmin2





        NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
        10-30-2026, 06:46 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by SEQadmin2, 11-12-2026, 07:59 AM
      0 responses
      27 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-05-2026, 09:25 AM
      0 responses
      35 views
      0 reactions
      Last Post SEQadmin2  
      Started by SEQadmin2, 11-03-2026, 11:54 AM
      2 responses
      56 views
      0 reactions
      Last Post kinpict.com  
      Started by SEQadmin2, 10-28-2026, 09:57 AM
      0 responses
      175 views
      0 reactions
      Last Post SEQadmin2  
      Working...