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  • liux
    Member
    • Mar 2009
    • 30

    #1

    TopHat question: prep_reads not found?

    I am new to tophat. so be gentle.

    When I run tophat with a test yeast data set, I got an error message after "Checking reads". after 3 lines of reads info, it says:


    Code:
    [FONT="Courier New"]
    ~/$ bin/tophat '/path/to/bowtie/indexes/yeast' '/path/to/sequence/seq.fastq' 
    ...
    ...
    [Mon Aug 17 15:53:01 2009] Checking reads
    	seed length:	 33bp
    	format:		 fastq
    	quality scale:	 --phred33-quals
    	[FAILED]
    Error: prep_reads not found on this system.  Did you forget to include it in your PATH?
    [/FONT]
    Could someone help me to figure out what I did wrong?
  • liux
    Member
    • Mar 2009
    • 30

    #2
    Whoops, prep_reads is another program in tophat that is needed. once I add '/home/user/bin' in the PATH, it worked.

    Comment

    • mortnyc
      Junior Member
      • Oct 2009
      • 1

      #3
      Hey there, I too have the same problem i'm ot sure to which path am I gonna have to add that '/home/user/bin'?

      Comment

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