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denovo assembly
explanation about denovo asembly
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Assembling short reads to create full-length sequences . de-novo assemblies are orders of magnitude slower and more memory intensive than mapping assemblies. -
de novo is Latin, basically meaning "from new". So a de novo assembly is without knowing anything about the answer - compared to a reference guided assembly where you have a simple genome for guidance.Comment
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I love that such an incomplete question (phrase) got two thoughtful responses.Comment
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His/her ISP may be charging by the word to access the internet
Three posts (so far) by that user have less than 5 words each.
Originally posted by mgogol View PostComment
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Ha ha, good call.
On biostars, somebody would have deleted or closed it. I suppose there's a place for both approaches.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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