Chimeric reads hard clipping
Hello, everyone. I got a question without answer after a long exhaustive liaterature search. I am using multiple displacement amplification (MDA) to amplify my samples, followed by 454 sequencing. MDA is well known for the generation of chimera, and therefore chimeric reads. In my dataset, nearly 30% reads are chimeric, estimated by Bowtie 2 aligner. I wonder whether there are some tools can retrieve the mapped domains of chimeric reads only from SAM output. If yes, I think this may break chimeric reads into single mappable reads.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM
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