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  • toots
    Junior Member
    • Oct 2012
    • 2

    #1

    Plotting distribution of allelic frequency under different coverage values

    I'm new to bioinformatics, and have an incomprehensible PI. Could someone clue me in on some of what is going on in the prompt below? For instance:
    1) What's "LD"?
    2) What do the different coverage values signify? How do I implement that in R?
    3) I have no idea what the RefAlleleCount, etc. bit means. Aren't all of those ratios the same anyhow?

    I realize I'm asking really broad, noobish questions, but a little context would be reeeaaally helpful. Thanks.

    " RNAseq reads covered 5,000 biallelic human autosomal SNPs (all are heterogeneous, assume they are not in LD). Please write simple R statements to plot the distribution of the alternative allelic frequency under sequencing coverage 2x, 5x, 10x, 20x, respectively. Also calculate the p-values with the hypothesis that there are no differential allelic expression for these three SNPs: (RefAlleleCount:AltAlleleCount) 1:4, 2:8, 4:16. "

    Edit: I've discovered that LD is linkage disequilibrium. Still fairly lost though.
    Last edited by toots; 10-22-2012, 08:36 AM.
  • chadn737
    Senior Member
    • Jan 2009
    • 392

    #2
    1) LD = Linkage Disequalibrium
    2) Coverage refers to the number of reads covering each bp
    3) RefAlleleCount = # of reads with the reference allele, AltAlleleCount = # reads with an alternative allele (i.e. not matching the reference).

    Comment

    • toots
      Junior Member
      • Oct 2012
      • 2

      #3
      Is there actually enough information in that prompt to do an analysis in R? I don't even know which "three SNPs" are being referred to. Is that implicit somehow?

      Comment

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