Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • interpretation of output from HTSeq

    I ran the four commands below.

    python -m HTSeq.scripts.count accepted_hits.sam Mh.gtf > count.txt
    samtools view accepted_hits.bam | python -m HTSeq.scripts.count - Mh.gtf > count.txt
    samtools view accepted_hits.bam | python -m HTSeq.scripts.count -m intersection-strict - Mh.gtf > count.txt
    samtools view accepted_hits.bam | python -m HTSeq.scripts.count -m intersection-nonempty - Mh.gtf > count.txt


    However, all of the outputs show the same result:

    no_feature 0
    ambiguous 0
    too_low_aQual 0
    not_aligned 0
    alignment_not_unique 409168


    With this result, I don't think I can move to the next step, DESeq.
    How could I make interpretation on this? Would you see what is wrong with my commands or result and what whould be fixed?

    Thank you in advance.
    Last edited by syintel87; 01-06-2013, 01:56 PM.

  • #2
    I solved this by choosing the appropriate option, -s -i -t.
    Sorry, but I have no idea about how to delete my post.
    Last edited by syintel87; 01-06-2013, 05:17 PM.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM
    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    18 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    22 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    16 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    47 views
    0 likes
    Last Post seqadmin  
    Working...
    X